THERMO SCIENTIFIC 00-4555-56 Invitrogen Accutase Enzyme Cell Detachment Bottle 100ML

( Brand: Thermo Scientific ), ( Manufacturer Part Number: 00-4555-56 ), ( Part Type: Cell ), ( Unit Type: Unit ), ( Country Of Origin: United States ), ( Intended Use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory )

Review THERMO SCIENTIFIC #00-4555-56 Invitrogen Accutase Enzyme Cell Detachment Medium Bottle 100ml New And Sealed

The Thermo Fisher Scientific 00-4555-56 Invitrogen Accutase Cell Detachment Reagent is a premium solution designed for effective and efficient cell detachment from various types of cell culture surfaces. This bottle contains 100 milliliters of the reagent, making it a suitable volume for large-scale experiments or multiple uses.

Accutase is a recombinant, animal-free formulation that contains hyaluronidase and collagenase type I and IV activities. These enzymes work synergistically to detach adherent cells from surfaces without damaging them. The reagent is ideal for use with primary cells, stem cells, and other sensitive cell types that require gentle handling during cell detachment.

The Accutase solution is ready-to-use and does not require any additional additives or supplements. It is formulated with a balanced calcium and magnesium concentration that maintains optimal cellular integrity during the detachment process. The pH level of the solution is carefully adjusted to ensure compatibility with a wide range of cell culture media.

The Thermo Fisher Scientific 00-4555-56 Invitrogen Accutase Cell Detachment Reagent comes in a sterile, translucent bottle with a conveniently located opening for easy dispensing. The bottle is designed to minimize the amount of reagent that comes into contact with the environment, thereby reducing the risk of contamination.

When using Accutase for cell detachment, it is recommended to incubate the cells with the reagent for 5 to 10 minutes at 37 C. During this time, the enzymes in the Accutase solution will gently break down the extracellular matrix, allowing the cells to detach from the surface. Once the cells have detached, they can be easily collected by gentle pipetting or filtration through a cell strainer.

Overall, the Thermo Fisher Scientific 00-4555-56 Invitrogen Accutase Cell Detachment Reagent is a versatile and effective solution for detaching adherent cells from various types of cell culture surfaces. Its gentle action and animal-free formulation make it an essential tool for researchers working with primary cells, stem cells, and other sensitive cell types.

A Comprehensive Analysis of Buying Thermo Fisher Scientific's Invitrogen Accutase: Pros, Cons, and Conclusion

The Thermo Fisher Scientific's Invitrogen Accutase is a widely used enzyme solution for detaching adherent cells from culture plates for further experiments. This analysis provides an in-depth examination of the benefits and drawbacks of purchasing this product.

1. Highly Efficient: Accutase effectively detaches adherent cells from various types of surfaces, including tissue culture plastic and glass, with minimal damage to the cells.

2. Versatile: It can be used for both primary and established cell lines, making it a valuable tool for researchers in various fields.

3. Reduced Trypsin Use: Accutase offers a gentler alternative to trypsin, which can be harsh on cells and lead to increased cell death.

4. Faster Detachment: Compared to trypsin, Accutase yields faster detachment times, saving valuable laboratory time.

5. Easy to Use: The solution comes ready-to-use, eliminating the need for extensive preparation and saving time.

1. Expensive: The cost per milliliter of Accutase is higher than that of trypsin, making it a more expensive option for cell detachment.

2. Limited Volume Options: The product is only available in larger volumes, which may not be suitable for smaller-scale experiments.

3. Potential Batch Variability: As with any biological reagent, there can be variability between batches, which may impact the consistency and reliability of results.

The Thermo Fisher Scientific's Invitrogen Accutase offers several advantages over traditional trypsin for cell detachment, including increased efficiency, gentler cell handling, faster detachment times, and versatility. However, its higher cost per milliliter and limited volume options may be considered drawbacks. Ultimately, the decision to purchase Accutase depends on the specific requirements and budget of your research project.

For researchers conducting large-scale experiments or working with delicate cell lines, the benefits of Accutase outweigh the costs. However, for smaller-scale experiments or those with limited budgets, trypsin may still be a more cost-effective option. In such cases, it is essential to carefully weigh the advantages and disadvantages before making a purchasing decision.

Details:

Cell Type Mammalian Cells. Accutase has been shown effective on: , creationists, vascular endothelial cells, hepatocytes, smooth muscle hepatocyte progenitors, primary chick embryo neuronal bone marrow stem adherent CHO and BHK macrophages, 293 L929 immortalized mouse testicular germ 3T3, Verso, COS, Heal, NT2, MG63, M24 A375 metastatic melanoma, gloom's U251 D54, HT1080 Sf9 insect cells. Cell Culture. Quantity: 1 bottle 100 mL per order.

part #: 00455556 prices

• $215.99-$2950.00

specifications labequipi:

• brand: Thermo Scientific
• mpn: 00-4555-56
• part type: Cell
• unit type: Unit
• country of origin: United States
• intended use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory

shipping labequipi:

• type: Calculated
• service: USPS Priority Mail
• cost: $0.00
• transit: 4-8 Days
• carrier: USPS
• class: Expedited

general labequipi:

• Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment
• condition: New

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• policy: Returns Accepted
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• time: 30 Days

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• # reviews: 718
• rating: 100.0%
• city: Winston-Salem, North Carolina

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offer labequipi:

• availability: In Stock
• sold: 0
• started: November 3, 2025
• options: Ship-to-home
• quantity: 5

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