
( Brand: Thermo Scientific ), ( Manufacturer Part Number: 044150.MD ), ( Country Of Origin: Germany ), ( Intended Use/discipline: Biological Laboratory, Microbiological Laboratory, Physical Laboratory )
The Thermo Scientific 044150.md 2,6-DiPhenyl-1-Picrylhydrazyl (DPPH) Radical Solution is a high-performance antioxidant reagent designed for the quantitative determination of antioxidant activity in various samples. This solution comes in a dark brown colored bottle, which is a characteristic of DPPH due to the presence of the stable free radical DPPH.
DPPH is a stable, purple-colored nitroxyl radical, which readily accepts electrons or hydrogen atoms from antioxidants. The antioxidant capacity of a sample is determined by the ability to reduce the DPPH radical to its colorless diphenylpicrylhydrazine form. The decrease in absorbance at 517 nm is directly proportional to the antioxidant activity of the sample.
This Thermo Scientific DPPH solution is supplied at a concentration of 95%, based on the absorbance at 517 nm against a blank solution. It is recommended for use within 3 months after opening, due to the gradual loss of radical activity over time. To maintain the stability of the solution, it should be stored in a cool, dark place, away from light and oxygen.
The DPPH solution is suitable for use in various applications, including the determination of antioxidant capacity in foods, beverages, cosmetics, pharmaceuticals, and biological samples. It is also useful for quality control and research purposes in the fields of food science, pharmacology, and biochemistry.
To prepare a working standard solution, dilute the DPPH solution with an appropriate solvent, such as methanol or ethanol. The optimal concentration of the working solution may vary depending on the specific sample and the sensitivity of the analytical method.
For precise and accurate results, it is important to follow the recommended protocols for sample preparation, dilution, and data analysis. The Thermo Scientific DPPH solution, along with the proper equipment and analytical techniques, enables researchers and analysts to assess the antioxidant activity of various samples with confidence and accuracy.
2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical used extensively in the field of organic chemistry and biochemistry for various applications, including antioxidant assays. Thermo Scientific is a reputable brand offering this reagent in a high-purity form (95%), with the product code 044150.MD. In this analysis, we will discuss the potential advantages and disadvantages of purchasing this DPPH reagent from Thermo Scientific.
Pros:1. High purity: Thermo Scientific's 044150.MD DPPH comes with a guaranteed minimum purity of 95%. This level of purity is essential for accurate and reproducible results in various applications.
2. Reputable brand: Thermo Scientific is a well-established brand with a strong reputation in the scientific community. Purchasing from a reputable supplier ensures the authenticity and quality of the product.
3. Convenient size: The 250 mg size of the Thermo Scientific DPPH vial is a common and convenient size for most laboratories, making it a cost-effective option for researchers.
Cons:1. Potential instability: DPPH is known to be an unstable compound, particularly when exposed to light or air. Proper storage conditions (in a dark, airtight container) are essential to maintain the integrity of the reagent.
2. Relatively high cost: While the Thermo Scientific 044150.MD DPPH is competitively priced compared to other brands, it may still be considered relatively expensive compared to some alternatives.
3. Hazardous: DPPH is classified as a hazardous substance due to its picric acid content. Proper handling and disposal procedures must be followed to ensure safety.
Conclusion:The Thermo Scientific 044150.MD 2,2-Diphenyl-1-picrylhydrazyl (DPPH) offers several advantages, such as high purity, a reputable brand, and a convenient size. However, the potential instability, relatively high cost, and hazardous nature of the compound are important considerations. Ultimately, the decision to purchase this DPPH reagent from Thermo Scientific should be based on the specific needs and resources of your laboratory.
Recommendation:If your laboratory requires a high-purity, reliable source of DPPH for antioxidant assays or other applications, the Thermo Scientific 044150.MD 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is an excellent choice. Ensure proper handling and storage procedures are followed to maintain the integrity of the reagent. If budget constraints are a concern, it may be worth exploring alternative brands or suppliers offering similar purity levels.
250mg bottle. Brand new and sealed.