
( Brand: Applied Biosystems ), ( Manufacturer Part Number: 0711-0013 ), ( Country/region Of Manufacture: United States )
The Applied Biosystems SPHERIS 5 RP-18 5 m, 30x4.6mm column is a high-performance reversed-phase liquid chromatography (LC) column designed for use with the SPHERIS 5 system. This column is manufactured by Brownlee Scientific, a leading supplier of chromatography columns, and is distributed by Applied Biosystems.
The SPHERIS 5 RP-18 5 m column features a 5 m particle size, which provides excellent efficiency and resolution, making it ideal for complex separations. The 30x4.6mm column dimension offers a good compromise between sample capacity and separation efficiency.
The stationary phase on this column is made up of 100% spherical, 5 m silica particles coated with a reversed-phase stationary phase material consisting of a C18 bonded phase. This stationary phase provides excellent retention and separation of a wide range of analytes, including small organic molecules and peptides.
The SPHERIS 5 RP-18 5 m column is packed in a stainless steel column body, which ensures durability and resistance to corrosion. The column is also equipped with a fused silica guard column, which protects the analytical column from particle contamination and provides an additional filtration step for the mobile phase.
This column is suitable for use with various mobile phases, including water, acetonitrile, methanol, and acetone, among others. It can be used in various LC modes, including normal-phase, reversed-phase, and ion-exchange modes, depending on the analytes and separation requirements.
The SPHERIS 5 RP-18 5 m column is compatible with most LC systems, including those manufactured by Applied Biosystems. It can be used for various applications, including method development, method validation, and routine analysis. The column is also suitable for use in various industries, including pharmaceuticals, biotechnology, and food and beverage industries, among others.
Overall, the Applied Biosystems SPHERIS 5 RP-18 5 m, 30x4.6mm column is a high-quality, versatile LC column that offers excellent separation efficiency, resolution, and durability. It is an essential tool for researchers and analysts working in various fields who require high-performance LC separations.
Applied Biosystems (AB) Brownlee Spheri-5 RP-18 5u 30x4.6mm columns are high-performance reversed-phase liquid chromatography (LC) columns designed for the separation and analysis of small molecules. Here are some potential pros and cons of buying these columns:
Pros:1. High Resolution and Efficiency: These columns offer high resolution and efficiency, which is essential for accurate and precise separation of complex mixtures. The small particle size (5 m) and the long length (30 cm) contribute to this high performance.
2. Wide Application Range: The Spheri-5 RP-18 columns are suitable for a wide range of applications, including protein and peptide purification, small molecule analysis, and method development. They can handle a broad range of pH and temperature conditions, making them versatile tools in the laboratory.
3. Excellent Reproducibility and Consistency: AB columns are known for their excellent reproducibility and consistency. This is crucial for accurate quantitative analysis and method development.
4. High Sample Loading Capacity: These columns have a high sample loading capacity, which allows for the analysis of larger sample volumes or more complex samples.
Cons:1. Cost: These columns can be quite expensive compared to other LC columns. The high price tag may be a significant consideration for some laboratories or researchers with limited budgets.
2. Fragile: The small particle size and long length of these columns make them more fragile than standard LC columns. Proper handling and care are essential to avoid damaging the column and ensuring its longevity.
3. Requires Specific Conditions: These columns may require specific conditions, such as a particular solvent system or gradient elution, to achieve optimal separation. This can limit their applicability to certain applications or require additional investment in solvents and other consumables.
In conclusion, Applied Biosystems Brownlee Spheri-5 RP-18 5u 30x4.6mm columns offer high performance, wide application range, and excellent reproducibility and consistency. However, they come with a high price tag and require specific conditions and careful handling.
If the research or application requires high resolution and efficiency, a wide range of applications, and accurate quantitative analysis, these columns may be a worthwhile investment. On the other hand, if the budget is limited or the application does not require the highest performance LC column, alternative options with lower prices and less stringent requirements may be more suitable. Ultimately, the decision to purchase these columns depends on the specific research goals, budget, and technical requirements of the laboratory.
# 1080-75, This sale is for two new Applied Biosystems Brownlee SPHERI-5, RP-18 5u, 30 x 4.6mm columns; 0711-0013. This sale is for two new Applied Biosystems Brownlee SPHERI-5, RP-18 5u, 30 x 4.6mm columns; 0711-0013, p/n: 0711-0013; Inc.