
( Brand: Thermo Scientific ), ( Manufacturer Part Number: 077998 ), ( Country Of Origin: United States ), ( Intended Use/discipline: Hplc Column )
The Thermo Scientific Accel-PEG 2.1, 100 mm, 3 m HPLC Column by Thermo Fisher Scientific is a high-performance liquid chromatography (HPLC) column designed for use in the separation and analysis of a wide range of compounds. This particular column, identified by the model number 077998, is part of the Accel-PEG (Polar Advantage) product line, which is known for its exceptional peak shape and resolution capabilities.
The column features a 100 mm length and a 2.1 mm inner diameter, making it well-suited for use in modern HPLC systems that employ smaller sample volumes and shorter run times. The particle size of the stationary phase is 3 microns, ensuring that the column can efficiently separate and resolve even the most complex mixtures of compounds.
The stationary phase of the column is composed of thermally bonded, porous, polar, and end-capped polystyrene divinylbenzene particles. The polar nature of the stationary phase allows for the effective separation of polar compounds, while the end-capping process minimizes the presence of silanol groups on the particle surface, reducing the potential for unwanted interactions between the stationary phase and analytes that contain functional groups such as amines or phenols.
The Accel-PEG 2.1, 100 mm, 3 m HPLC Column is capable of operating over a wide range of temperatures, with a recommended operating temperature range of 0-40 C. The column is also compatible with a range of mobile phases, including water, acetonitrile, methanol, and various buffers, making it a versatile tool for use in a variety of analytical applications.
The column is delivered in a protective sleeve and includes a filter and a pre-column to ensure that it is ready for use as soon as it is installed in an HPLC system. The Thermo Scientific Accel-PEG 2.1, 100 mm, 3 m HPLC Column is a high-quality, reliable, and versatile tool for the separation and analysis of a wide range of compounds in fields such as pharmaceuticals, biotechnology, food and beverage, and environmental science.
The Thermo Scientific Acclaim Polar Advantage C18 PA2 HPLC Column (077998) is a widely used stationary phase for high-performance liquid chromatography (HPLC) separations. This column is known for its excellent performance in separating polar and ionic compounds. In this analysis, we will discuss the pros and cons of investing in this Thermo Scientific column.
Pros:1. High Efficiency: The Thermo Scientific Acclaim Polar Advantage C18 PA2 HPLC Column has a 100 mm length and 3 m particle size, which provides high efficiency separations with excellent resolution and peak capacity.
2. Broad Application Range: This column is suitable for separating a wide range of polar and ionic compounds, including peptides, sugars, nucleotides, organic acids, and basic compounds.
3. Excellent Reproducibility: Thermo Scientific columns are known for their consistency and excellent reproducibility, ensuring reliable and accurate results.
4. Robust: The column can withstand harsh conditions and high back pressures, making it suitable for use with a wide range of mobile phases and solvent systems.
Cons:1. Expensive: The Thermo Scientific Acclaim Polar Advantage C18 PA2 HPLC Column is more expensive compared to other HPLC columns from various manufacturers.
2. Requires Proper Handling: Proper handling and storage conditions are essential to maintain the column's performance and lifespan.
3. Potential for Column Overloading: The high efficiency of the column can lead to column overloading, which may affect the separation efficiency and column lifespan.
Conclusion:The Thermo Scientific Acclaim Polar Advantage C18 PA2 HPLC Column (077998) is an excellent investment for laboratories working with a wide range of polar and ionic compounds, requiring high efficiency separations. Despite its high cost, the column's broad application range, excellent reproducibility, and robustness make it a valuable asset in many research and quality control environments. It is essential to follow proper handling and storage conditions to ensure optimal performance and column lifespan.
Recommendation:If you are working with polar and ionic compounds and require high-performance separations, the Thermo Scientific Acclaim Polar Advantage C18 PA2 HPLC Column (077998) is a recommended investment. Ensure that you follow the manufacturer's guidelines for handling and storage to maintain the column's efficiency and lifespan.
Used Good Working Condition, Thermo Acclaim Polar Advantage II C18 PA2 2.1 x 100mm 3um HPLC Column, Part No: 077998, Its a reversed-phase column designed to resolve both polar and non-polar compounds in single run. It features an amide polar-embedded stationary phase that provides unique selectivity complementary to conventional C18 columns. Stable Performance: The polar-embedded phase allows for operation over a wider range of conditions than conventional reversed-phase columns while maintaining stability at high and low pH.