PACBIO 100-426-900 Sequel Genome Sequencing
( Brand: Pacbio ), ( Manufacturer Part Number: 100-426-900 ), ( Intended Use/discipline: Biological Laboratory )
Review PACBIO Sequel Genome Sequencing System
The PacBio Sequel System from Pacific Biosciences is a revolutionary platform for long-read sequencing, offering high-quality and highly accurate genome sequences. This specific product, 100-426-900, is a Sequel System I/O SMRT Cell 8M Pack, designed to deliver large amounts of data for comprehensive genome analysis.
Each SMRT Cell contains millions of zero-mode waveguides, tiny nanometer-scale chambers where DNA molecules are captured and sequenced using PacBio's Single Molecule Real-Time (SMRT) technology. This technology allows for the direct observation and measurement of individual DNA molecules as they are being read, resulting in long, continuous reads that span an average of 10-20 kilobases.
The 100-426-900 SMRT Cell Pack provides researchers with the capacity to generate over 100 Gb of raw sequence data per run. These long reads offer significant advantages over short-read technologies, enabling the detection of structural variants, complex repeat regions, and full-length RNA transcripts that are often missed with short-read data.
Furthermore, the PacBio Sequel System offers high-accuracy base calling, thanks to its real-time error correction algorithms. With consensus base calling accuracy exceeding Q40 Phred quality score, researchers can trust the data they generate to be highly reliable and of the highest quality.
In summary, the PacBio 100-426-900 Sequel System I/O SMRT Cell 8M Pack is an essential tool for researchers seeking to generate high-quality, long-read genomic data for comprehensive genome analysis, structural variant detection, and RNA sequencing applications. With its large capacity and high-accuracy base calling, the PacBio Sequel System sets a new standard for genomic sequencing and analysis.
PacBio Sequel Genome Sequencing: Pros and Cons
PacBio Sequel genome sequencing is a long-read sequencing technology developed by Pacific Biosciences. Long-read sequencing is advantageous in providing more complete and accurate genome sequences compared to short-read sequencing technologies. Here are some pros and cons of using PacBio Sequel genome sequencing:
Pros:1. Long read length: PacBio Sequel generates reads up to 60 kb in length, which is much longer than short-read sequencing technologies. Long reads enable the detection of structural variants, repetitive regions, and complex genomic structures that are difficult to analyze with short reads.
2. High accuracy: PacBio Sequel uses a single molecule real-time (SMRT) sequencing approach, which provides high accuracy with error rates as low as 1 in 10,000 bases. This accuracy is crucial for identifying rare variants and mutations.
3. Real-time base calling: PacBio Sequel provides real-time base calling, which enables the identification of errors and artifacts during the sequencing process. This feature allows for improved data quality and increased efficiency.
4. Flexibility: PacBio Sequel can be used for various applications, including whole-genome sequencing, targeted sequencing, and transcriptome analysis. This versatility makes it a valuable tool for researchers and clinicians.
Cons:1. Cost: PacBio Sequel is a more expensive sequencing technology compared to short-read sequencing. The cost per base is higher, and the cost of consumables and instrumentation is also significant.
2. Throughput: PacBio Sequel has a lower throughput compared to short-read sequencing technologies. The instrument can generate around 10 Gb of data per SMRT cell, which may not be sufficient for large-scale projects.
3. Data analysis: Analyzing PacBio Sequel data can be challenging due to the complexity and size of the reads. Special software and computational resources are required to process and analyze the data effectively.
4. Limited availability: PacBio Sequel is not as widely available as short-read sequencing technologies. Access to the instrument and consumables may be limited, and there may be a longer turnaround time for sequencing projects.
Conclusion:PacBio Sequel genome sequencing is a powerful tool for generating high-quality, long-read genomic data. Its advantages include the ability to detect structural variants and complex genomic structures, high accuracy, real-time base calling, and flexibility. However, its disadvantages include a higher cost per base, lower throughput, complex data analysis, and limited availability.
Recommendation:If you require high-quality, long-read genomic data for complex genomic projects, PacBio Sequel may be the best choice. Its accuracy and ability to detect structural variants make it a valuable tool for researchers and clinicians. However, if cost and throughput are a major concern, short-read sequencing technologies may be a more practical option. Ultimately, the choice between PacBio Sequel and short-read sequencing will depend on the specific needs and resources of your project.
Details:
Comes as pictured made in 2019. I am told that his guy works great and there is nothing wrong with it. Shipping charge is flat rate 1,000.00. We just pulled out of a local lab here in the San Francisco bay area.
part #: 100426900 prices
- $5255.99-$9999.99
specifications labequipi:
- brand: Pacbio
- mpn: 100-426-900
- intended use/discipline: Biological Laboratory
general labequipi:
- condition: Used
- Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment
offer labequipi:
- best offers: True
- availability: In Stock
- started: July 1, 2025
- quantity: 1
- options: Ship-to-home
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- city: Patterson, California
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- type: Fixed
- cost: $1000.00
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- class: Standard
- carrier: USPS
- service: USPS Ground Advantage
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- other
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returns labequipi:
- policy: Returns Accepted
- time: 30 Days
- paid by: Buyer
- method: Money back
