
( Brand: Agilent ), ( Manufacturer Part Number: 101991-100 ), ( Model: XFE96 ), ( Part Type: Analyzer )
The Agilent Seahorse XFe96 Extracellular Flux Analyzer is a state-of-the-art research tool designed for measuring real-time metabolic responses of live cells. This analyzer, specifically the model number 101991-100, is manufactured by Seahorse Bioscience and comes with an Agilent warranty for added peace of mind.
The XFe96 Analyzer features a 96-well microplate format, enabling high-throughput analysis of multiple samples in a single run. The instrument is based on the Seahorse XFe Technology, which uses real-time, non-invasive sensors to measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in real-time. These parameters provide valuable insights into cellular bioenergetics, glycolysis, oxidative phosphorylation, and other metabolic processes.
The XFe96 Analyzer is equipped with a user-friendly software interface, Seahorse Wave, which simplifies data acquisition, analysis, and interpretation. The software accommodates various experimental designs, including the standard Seahorse Mito Stress Test and Glycolysis Stress Test protocols. Additionally, users can customize their experiments, adding up to four different injection ports for adding reagents at specific timepoints to probe cellular metabolic responses.
The instrument's design ensures precise and reproducible results, as it maintains a controlled temperature (37 C) and pH (7.4) environment, minimizing the impact of external factors on the measurements. The XFe96 Analyzer also includes a built-in shaking mechanism to optimize reagent mixing and ensure even distribution.
The Agilent Seahorse XFe96 Extracellular Flux Analyzer is ideal for researchers in various fields, including pharmaceutical, biotechnology, academic, and government organizations, to study cellular metabolism, drug discovery, and disease mechanisms. The instrument's versatility, high-throughput capabilities, and real-time data acquisition make it an indispensable tool for understanding the metabolic landscape of cells and their responses to various stimuli.
The Agilent Seahorse XFe96 Extracellular Flux Analyzer is a sophisticated instrument used for measuring real-time metabolic parameters in live cells. This technology, based on Seahorse Bioscience's Extracellular Flux (ECF) analysis, enables researchers to study metabolic processes such as glycolysis, oxidative phosphorylation, and cellular respiration. Here are some pros and cons of purchasing a Agilent Seahorse XFe96 Extracellular Flux Analyzer:
Pros:1. Real-time metabolic analysis: The XFe96 can measure real-time metabolic data for multiple wells, providing insight into cellular respiration and glycolysis under different conditions.
2. High-throughput: This instrument can analyze multiple parameters in a single run, reducing the time and resources required for traditional methods.
3. User-friendly: The Seahorse Wave software is intuitive and easy to use, making it suitable for researchers with varying levels of experience.
4. Integrated sensors: The XFe96 includes sensors for pH, oxygen, and extracellular acidification rate (ECAR) and ATP production rate (OCR), making it a versatile tool for studying cellular metabolism.
5. Compatible with various cell types: The XFe96 can be used for a wide range of cell types, including adherent, suspension, and primary cells.
Cons:1. High cost: The instrument and consumables can be expensive to purchase and maintain, making it a significant investment for some research groups.
2. Requires specialized training: Operating the XFe96 requires a good understanding of the technology and the biology being studied.
3. Limited sample volume: Each well has a limited sample volume, which might not be suitable for experiments requiring large amounts of cells or reagents.
4. Potential for experimental variability: Factors such as temperature, pH, and oxygen levels can impact the results, requiring careful control and optimization.
5. Limited number of measurements per run: Each run can only provide data for a limited number of measurements, which may not be sufficient for comprehensive studies.
In conclusion, the Agilent Seahorse XFe96 Extracellular Flux Analyzer is a powerful tool for studying cellular metabolism in a high-throughput and real-time manner. Its ease of use, versatility, and ability to provide valuable insights into metabolic processes make it an attractive option for researchers in various fields. However, its high cost, limited sample volume, and requirement for specialized training are important considerations.
If your research group has the resources and expertise to utilize the XFe96 effectively, it can offer significant advantages in understanding cellular metabolism and its role in various biological processes. However, if the cost and requirements are prohibitive, alternative methods or instruments may be more suitable for your research goals. Ultimately, the decision to invest in the Agilent Seahorse XFe96 Extracellular Flux Analyzer should be based on a thorough evaluation of its benefits and limitations in the context of your research objectives.
Agilent surplus, it has been repackaged by and labeled as un refurbished. Agilent Seahorse, Seahorse XFe96, XFe96 analyzer, extracellular flux metabolic analysis, 96-well OCR ECAR, XF96, real-time cell metabolism, mitochondrial function assay, glucose's oxygen consumption rate, edification nonnarcotics, live high-throughput XF Bioscience, assay system: Science Extracellular Flux Analyzer w/ Monitor; SN:410188. We endeavor to source functional and well-maintained equipment. CONDITION This product is used and unconditionally guaranteed to meet your full satisfaction upon receipt.