INVITROGEN 12183018A Thermo Fisher Purelink Rna Mini Kit 50

( Brand: Invitrogen ), ( Manufacturer Part Number: 12183018A ), ( Unit Type: Unit ), ( Unit Quantity: 50 Reactions ), ( Country Of Origin: United States ), ( Intended Use/discipline: Biological Laboratory, Microbiological Laboratory ), ( California Prop 65 Warning: For Research Use Only. Not For Clinical Or Diagnostic Use. )

Review INVITROGEN Thermo Fisher Purelink Rna Mini Kit 50 Prep

The Invitrogen PureLink RNA Mini Kit (Catalog Number: 12183018A) from Thermo Fisher Scientific is a versatile and high-performing solution for isolating total RNA from various types of samples. This kit is particularly well-suited for small-scale RNA extractions, making it an ideal choice for research laboratories, molecular biology applications, or any setting where RNA isolation is required.

The PureLink RNA Mini Kit employs a unique magnetic bead technology that allows for efficient and selective binding of RNA, while effectively removing contaminating DNA, proteins, and phenol. These magnetic beads are coated with a proprietary silane-coated spin column matrix, which facilitates the binding, washing, and elution steps of the RNA purification process.

The kit includes all necessary reagents and supplies for RNA isolation, including lysis buffer, binding buffer, ethanol, and magnetic beads. It also comes with a spin column for easy and convenient RNA purification. The provided RNase-free water is suitable for use as a diluent or elution buffer.

The PureLink RNA Mini Kit is compatible with a wide range of starting materials, such as animal and plant cells, fungi, bacteria, and cultured cells. It can be used with various lysis methods, including direct lysis, spin column lysis, and guanidine thiocyanate lysis. Moreover, the kit is designed to preserve RNA integrity, ensuring that your precious RNA samples remain intact throughout the isolation process.

1. High purity: The kit efficiently removes impurities, resulting in RNA that is free from contaminating DNA, proteins, and phenol.

2. Easy and quick: The magnetic bead technology and user-friendly spin column design make RNA isolation a fast and straightforward process.

3. Broad compatibility: The kit can be used with various starting materials and lysis methods.

4. High yield: The kit provides excellent RNA recovery, ensuring that you obtain sufficient material for downstream applications.

5. Integrity preservation: The RNA remains intact throughout the isolation process, maintaining its quality and functionality.

In conclusion, the Invitrogen PureLink RNA Mini Kit (Catalog Number: 12183018A) is an indispensable tool for any laboratory that requires RNA isolation. With its high purity, ease of use, broad compatibility, high yield, and integrity preservation capabilities, this kit is an excellent choice for a wide range of research and molecular biology applications.

Pros and Cons of Buying Invitrogen PureLink RNA Mini Kit (Thermo Fisher Scientific, Cat. No. 12183018A)

The Invitrogen PureLink RNA Mini Kit by Thermo Fisher Scientific is a widely used RNA purification kit for lab-scale RNA isolation from various types of samples. This article will discuss the key advantages and disadvantages of using this particular kit for your RNA extraction needs.

1. High yield and purity: The PureLink RNA Mini Kit offers high RNA yield and purity, making it an excellent choice for downstream applications such as cDNA synthesis, sequencing, and quantitative PCR (qPCR).

2. Versatility: This kit can be used to isolate RNA from various sources, including plant, fungal, bacterial, and animal tissues, as well as cultured cells.

3. Simple protocol: The RNA isolation process using this kit is straightforward and can be completed within a few hours. It involves a one-step lysis and binding procedure, which saves time and effort.

4. Robust: The kit has a proven track record of reliability and robustness, ensuring consistent RNA isolation results.

5. Comprehensive: The kit includes all necessary reagents for RNA isolation, eliminating the need to purchase additional components.

1. Cost: The Invitrogen PureLink RNA Mini Kit can be relatively expensive compared to other RNA purification kits on the market.

2. Potential for carryover contamination: As with any RNA isolation kit, there is a risk of carryover contamination, especially if not following proper precautions during the isolation process.

3. Limited scale: While suitable for lab-scale RNA isolation, this kit may not be the best choice for larger-scale RNA extractions.

4. Potential for DNase inactivation: The DNase treatment step in the kit can be challenging to optimize, and in some cases, may not completely remove all DNA contamination.

The Invitrogen PureLink RNA Mini Kit is a reliable and versatile choice for lab-scale RNA isolation with high yield and purity. Its simplicity, robustness, and compatibility with a wide range of sample types make it a popular choice in research laboratories. However, its cost and potential for carryover contamination or incomplete DNase treatment are considerations that should be weighed against the benefits.

If you require a lab-scale RNA purification kit with high yield and purity, the Invitrogen PureLink RNA Mini Kit is an excellent choice. However, if cost is a significant concern, consider exploring alternative, more budget-friendly options. Additionally, be sure to follow proper precautions to minimize the risk of carryover contamination and carefully optimize the DNase treatment step for best results.

Details:

High-quality total RNA can be obtained from mini to midi-prep amounts of starting material with just trace residual genomics DNA contamination. The kit includes RNA's-free lases and wash solutions that protect RNA from Rinses while liberating the DNA, proteins, other cellular debris. 12185010 for easy removal of residual DNA. Simplified, non-toxic RNA purification.

Use isolated RNA in most applications without the need for further Danes treatment very low minimal residual DNA carry over. Both the 'on-column' and post-RNA purification workflows are options available with PureLink Danes Set. Treating with Danes while 'on-column' is easier and allows higher RNA recovery than treating after the has been isolated. Isolate more RNA than competitor mini spin columns: up to 1000of total from one column.

However, some applications such as gene expression analysis by qr-PCR without intro-spanning primers or working with samples from organisms very small no intros may require more complete removal of residual contaminating DNA. The Homogenize is especially effective for clarifying particulates from plant tissues. Easy, optional, on-column Danes treatment for sensitive applications. This means that users can process both small and large sample sizes with the same RNA isolation kit.

The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. Extra-large binding capacity enables rapid RNA purification using standard laboratory equipment. The advanced PureLink RNA Mini spin column design allows for maximum sample input 200 mg of tissue and recovery up to 1000 g. Process sample inputs from 5 200 mg of animal/plant tissue or 0.5 x 106 to 108 culture cells with one spin column in 20 minutes.

The PureLink RNA Mini Kit is recommended for use with the Homogenize, designed to homogenize cell or tissue lasts via gentrification, prior nucleic acid purification. Flexible optional on-column or post-isolation Danes treatment is available with PureLink Set Cat. The PureLink Danes Set can also be used to remove residual DNA from RNA that has been previously purified. The PureLink RNA Mini Kit columns are highly efficient for isolating high quality total while removing the majority of genomics DNA.

The PureLink RNA Mini Kit utilizes non-toxic quinidine- lases buffer to protect the during isolation steps.

part #: 12183018a prices

• $269.99-$279.99

specifications labequipi:

• brand: Invitrogen
• mpn: 12183018a
• unit type: Unit
• unit quantity: 50 Reactions
• country of origin: United States
• intended use/discipline: Biological Laboratory, Microbiological Laboratory
• california prop 65 warning: For Research Use Only. Not For Clinical Or Diagnostic Use.

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• condition: New
• Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment

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• # reviews: 1

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• started: March 11, 2026

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