
( Brand: Bio-rad ), ( Manufacturer Part Number: 1620239 ), ( Part Type: Filter ), ( Country/region Of Manufacture: United States ), ( Intended Use/discipline: Biological Laboratory, Immunology, Medical Laboratory, Microbiological Laboratory )
The Bio-Rad 1620239 Immun-Blot PVDF Filter Papers are a essential component in the protein transfer step of Western Blotting experiments. These filter papers, also known as Trans-Blot Turbo Transfer PVDF Membranes, are pre-cut to a convenient size of 13 cm x 10 cm, making them ideal for standard Western Blotting procedures.
Each pack contains 50 individual filter papers, ensuring a sufficient supply for multiple experiments. The PVDF (Polyvinylidene Difluoride) material used in these filter papers offers several advantages. PVDF is a hydrophobic and highly porous membrane, allowing for efficient and consistent protein transfer from the gel to the membrane during the blotting process. Moreover, PVDF membranes are known for their superior binding capacity and durability, making them an excellent choice for the detection and analysis of a wide range of proteins.
The Immun-Blot PVDF Filter Papers from Bio-Rad are pre-equilibrated with methanol and water to ensure optimal transfer conditions. This eliminates the need for time-consuming and laborious pre-treatment steps, streamlining the Western Blotting workflow. Additionally, these filter papers are compatible with various transfer buffers and can be used with both vertical and semi-vertical transfer systems.
Each filter paper is sandwiched between two thin, microporous cellulose sheets. These sheets not only facilitate the transfer of proteins but also serve as a protective layer, preventing the membranes from being damaged during the transfer process. Once the transfer is complete, the filter papers can be easily removed, leaving the PVDF membrane ready for the subsequent steps of Western Blotting, such as blocking, probing with primary and secondary antibodies, and detection.
In summary, the Bio-Rad 1620239 Immun-Blot PVDF Filter Papers are a high-quality, reliable, and versatile choice for the protein transfer step of Western Blotting experiments. With their efficient transfer, superior binding capacity, and ease of use, these filter papers save researchers valuable time and resources while ensuring consistent and accurate results.
Bio-Rad Laboratories' 1620239 Immun-Blot PVDF Filter Paper Sandwiches (162-0239, 50/pk) are a popular choice for Western Blotting experiments in laboratories. In this analysis, we will discuss the advantages and disadvantages of purchasing these filter papers to help researchers make informed decisions.
Pros:1. High-quality PVDF membranes: The PVDF membranes in these filter papers offer excellent binding capacity for proteins and are known for their consistency and reliability.
2. Convenient sandwiched design: The pre-cut filter papers are already sandwiched between backing and protective layers, making the setup process more straightforward and time-efficient.
3. Consistent size and uniformity: The standard size and consistent thickness of the filter papers facilitate easy handling and consistent blotting results.
4. Wide compatibility: These filter papers are compatible with various electrophoresis systems and transfer buffers.
5. Long shelf-life: Properly stored, these filter papers can maintain their quality for an extended period, reducing the need for frequent replacements.
Cons:1. Cost: The price of these filter papers might be higher compared to other options, which could be a significant concern for laboratories with limited budgets.
2. Limited application: While widely used for Western Blotting, these filter papers may not be suitable for all types of applications or experimental setups.
3. Disposal considerations: Proper disposal of the used filter papers and backing layers should be followed to ensure safety and compliance with regulations.
Conclusion:Bio-Rad 1620239 Immun-Blot PVDF Filter Paper Sandwiches (162-0239, 50/pk) offer numerous benefits, including high-quality membranes, convenience, and consistency. However, their cost might be a disadvantage for some laboratories. When considering purchasing these filter papers, researchers should weigh the advantages against the potential costs and ensure that they align with their experimental needs.
Recommendation:If the advantages of using Bio-Rad 1620239 Immun-Blot PVDF Filter Paper Sandwiches (162-0239, 50/pk) outweigh the disadvantages for a researcher's specific application, they should consider purchasing these filter papers to benefit from their high-quality membranes, time-saving design, and consistent results.
Please see pictures for details on condition. TWO 2 PACKS, 50 PER PACK, Bio-Rad Immun-Blot PVDF/Filter Paper Sandwiches #1620239, 162-0239, per pack x 2 packs 100 total, 8.5 13.5 cm, pre cut reassembled blotting membrane/filter paper sandwich for unbolting. SHIPPING: - Flat Rate Shipping on most items is for the lower 48 USA States only. Internal packs still sealed.
New, Open Box. Fits Criterion and XT precast gels. ONLY receive what is pictured.