THERMO SCIENTIFIC 164564 Acclaim Pepmap Nanoviper Um 2-CM C18
( Brand: Thermo Scientific ), ( Manufacturer Part Number: 164564 ), ( Country Of Origin: United States )
Review THERMO SCIENTIFIC Acclaim Pepmap 100 Nanoviper Um Cm C18 Qty
The Thermo Scientific Accel-MS PEPmap NANOViper UM C18 analytical column is a high-performance reversed-phase chromatography column specifically designed for use in ultra-performance liquid chromatography (UPLC) and nano-flow liquid chromatography (nanoLC) systems. This column is an essential tool for the separation and analysis of complex peptide mixtures, particularly in the field of proteomics and mass spectrometry-based proteomic research.
The Accel-MS PEPmap NANOViper UM C18 column features a unique particle size of 1.7 m, which provides ultra-high resolution and high-speed separations. The column's 75 m i.d. x 150 mm length allows for efficient and rapid separations of peptides and proteins with nanoflow gradients. The C18 stationary phase is composed of a porous core with a surface area of 300 m2/g and a pore size of 100 , ensuring excellent retention and separation efficiency for a wide range of peptides.
The column's bead surface chemistry is also optimized for use with UPLC and nanoLC systems. The C18 stationary phase is bonded to the silica particles via a stable, covalent bond, ensuring excellent stability and compatibility with a wide range of mobile phases. The column's surface is also hydrophilic, reducing non-specific interactions with the column and increasing sample loading capacity.
The Thermo Scientific Accel-MS PEPmap NANOViper UM C18 column is ideal for use in proteomic research applications, such as peptide mapping, protein identification, and quantitation. It is also suitable for the analysis of small molecules and other analytes that require high-resolution separations.
This column is designed to be used with a variety of mass spectrometry systems, including quadrupole, orbitrap, and time-of-flight (TOF) mass spectrometers. It is also compatible with a range of mobile phases, including water, acetonitrile, methanol, and various buffers. The column is packaged in a protective sleeve and comes with a filter and a stainless steel column nut for easy installation in most UPLC and nanoLC systems.
In summary, the Thermo Scientific Accel-MS PEPmap NANOViper UM C18 analytical column is a high-performance reversed-phase chromatography column designed for use in UPLC and nanoLC systems. Its unique particle size, optimized surface chemistry, and high-resolution separations make it an essential tool for proteomic research applications, particularly in the field of mass spectrometry-based proteomics.
The Thermo Scientific 164564 Acclaim PepMap Nanoviper UM-C18 column is a popular choice for liquid chromatography (LC) separations, particularly in the field of peptide mapping and proteomics. Here are some pros and cons to consider before making a purchase:
Pros:1. High-Performance Reversed-Phase LC: The UM-C18 stationary phase offers excellent separation efficiency and high resolution, making it suitable for complex peptide mixtures.
2. Nanoscale Porosity: The nanoscale porosity of the particle size (1.9 m) provides increased surface area and improves sensitivity for detecting low-abundance peptides.
3. C18 Stationary Phase: The C18 (cyano) stationary phase is commonly used for reversed-phase LC separations due to its hydrophobic nature, which allows for effective retention and separation of nonpolar compounds.
Cons:1. Cost: The Thermo Scientific 164564 Acclaim PepMap Nanoviper UM-C18 column is a high-performance and expensive LC column.
2. Requires Specific Conditions: The column may require specific conditions, such as a particular mobile phase or column temperature, to ensure efficient separations.
3. Limited Sample Capacity: Due to its small particle size and high efficiency, the column has limited sample capacity, which may require fraction collection or other sample preparation methods.
In conclusion, the Thermo Scientific 164564 Acclaim PepMap Nanoviper UM-C18 column offers excellent separation efficiency and high resolution for complex peptide mixtures. However, its high cost and limited sample capacity may be a concern for some researchers. If the benefits of high-performance LC separations outweigh the costs, then this column is an excellent choice for peptide mapping and proteomics applications.
Recommendation:If you require a high-performance LC column for peptide mapping and proteomics, particularly for complex peptide mixtures, the Thermo Scientific 164564 Acclaim PepMap Nanoviper UM-C18 column is a recommended option. Consider the cost and sample capacity limitations when planning your research budget and sample preparation methods.
Details:
Acclaim PepMap 100. Inventory Location. C18, 5um, 100A. BUYER PAYS /6RETURN SHIPPING.
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part #: 164564 prices
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- brand: Thermo Scientific
- mpn: 164564
- country of origin: United States
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- condition: Used
- Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment
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- # reviews: 25177
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