
( Brand: Waters ), ( Manufacturer Part Number: 186004742 ), ( Country Of Origin: Ireland ), ( Intended Use/discipline: Biological Laboratory, Dental Laboratory, Microbiological Laboratory )
The Waters Acquity UPLC BEH Amide column 2.1 x 150 mm, 1.7 m is a high-performance reversed-phase column specifically designed for the separation of basic and neutral amide-containing compounds. This column is part of the Acquity Ultra Performance LC (UPLC) system, which delivers superior separation and enhanced sensitivity compared to traditional high-performance liquid chromatography (HPLC) systems.
The BEH Amide column features a unique stationary phase comprised of porous particle technology, which provides increased loading capacity, faster analysis times, and improved efficiency. The stationary phase consists of glycosylated amide bonded phases, which offer excellent selectivity and retention for basic and neutral amides. The column is capable of handling mobile phase pH ranges from 1.8 to 10.0, making it versatile for a wide range of applications.
The column dimensions are 2.1 mm x 150 mm with a particle size of 1.7 m. Its small particle size leads to increased efficiency and faster analysis times. The narrow bore size of 2.1 mm provides better column efficiency, increased sensitivity, and reduced solvent consumption.
The BEH Amide column is ideal for use in the analysis of peptides and proteins, particularly those containing amide bonds. It can be used in various applications such as the analysis of glycosylated peptides and proteins, in drug discovery and development, and in the analysis of complex biological samples.
The column is easy to use and can be integrated with various UPLC systems and autosamplers. It is also compatible with various detection techniques such as UV, fluorescence, and mass spectrometry. The BEH Amide column is known for its robustness and reliability, ensuring consistent and reproducible results.
In summary, the Waters Acquity UPLC BEH Amide column 2.1 x 150 mm, 1.7 m is a versatile and high-performance reversed-phase column designed for the separation of basic and neutral amide-containing compounds. Its small particle size, narrow bore size, and unique stationary phase make it an efficient and effective tool for various applications in the analysis of peptides, proteins, and complex biological samples.
a. High Selectivity: This column is designed specifically for glycan analysis, ensuring excellent separation and high selectivity for various glycan structures.
b. Efficient: With a particle size of 1.7 m, the column offers fast analysis times, making it an ideal choice for high-throughput glycan analysis.
c. Reliable: Thermo Scientific is a trusted brand in the field of analytical science, and the Accuity UPLC Glycan Amide Column is known for its robustness and reliability.
d. Compatibility: The column is compatible with common UPLC systems, making it a versatile option for laboratories already invested in this technology.
e. Consistent Performance: The column delivers consistent results, ensuring accurate and reliable glycan analysis.
2. Cons:a. Cost: This specialized column can be more expensive than general-purpose UPLC columns, which may be a deterrent for some laboratories with limited budgets.
b. Limited Sample Capacity: The column may not have the same sample capacity as larger, less specialized columns, which could require more frequent column changes.
c. Requires Specific Mobile Phases: To achieve optimal results, the column may require specific mobile phases, adding to the overall cost and complexity of the analysis.
3. Conclusion:The Thermo Scientific Accuity UPLC Glycan Amide Column 2.1 x 150 mm, 1.7 m is an excellent choice for laboratories focusing on glycan analysis due to its high selectivity, efficient analysis times, reliability, and consistent performance. However, its relatively high cost, limited sample capacity, and requirement for specific mobile phases should be considered before making a purchase.
4. Recommendation:If your laboratory focuses on glycan analysis and requires high-performance, specialized columns, the Thermo Scientific Accuity UPLC Glycan Amide Column is a recommended option. Ensure that your UPLC system is compatible with the column and budget for the additional costs associated with its use. If budget is a concern, consider alternative, less expensive columns for general glycan analysis or consider investing in this column as part of a larger investment in glycan analysis technology.
Chromatography Mode: HILIC Hydrophilic Interaction LC. Product Line: ACQUITY UPLC. These columns utilize BEH Bridged Ethylene Hybrid Amide chemistry, delivering excellent retention, resolution, for hydrophilic interaction chromatography HILIC. Specifications.
Brand: Waters. Pore Size: 130. Part Number P/N: 186004742. Column Chemistry:Glycan BEH Amide.
Country of Manufacture: Made in Ireland. The Waters ACQUITY UPLC Glycan BEH Amide Columns are high-performance chromatography columns designed specifically for glycan analysis using systems. Particle Size: 1.7 m. Ideal for laboratories seeking consistent performance across multiple analyses or long-term supply.
Suitable for pharmaceutical, biotech,clinical research, and analytical laboratories. Waters 186004742 Acquity UPLC Glycan BEH Amide Column 2.1mm X 150. Condition: New.