
( Brand: Waters ), ( Manufacturer Part Number: 186009898 ), ( Country/region Of Manufacture: Ireland )
Introducing the WATERS 186009898 XBridge Premier Peptide BEH C18 130 2.5 x 4.6mm LC Column, a premium chromatography solution designed for the most demanding peptide separations. This column is engineered to deliver superior separation efficiency, resolution, and reproducibility, making it an ideal choice for laboratories and research institutions working on complex peptide mixtures.
The XBridge Premier Peptide BEH C18 stationary phase features spherical, 130 porous particles, which provide an extensive surface area and optimized pore size distribution. This design enhances the column's ability to retain and separate peptides effectively, ensuring excellent peak shape and high sensitivity. The C18 stationary phase is also bonded using a robust, stable silane, ensuring long-term column performance and excellent column efficiency.
Measuring 2.5 x 4.6mm, this XBridge Premier Peptide BEH C18 LC column offers an ideal balance between sample loading capacity and separation efficiency. With a length of 50mm, it provides ample column length for efficient separation of even the most complex peptide mixtures.
The XBridge Premier Peptide BEH C18 column is designed to operate under a wide range of mobile phase conditions, making it a versatile tool for various applications. It is compatible with aqueous and organic mobile phases, and can be used with a variety of gradient elution methods. The column's robustness and excellent chemical resistance to acids, bases, and organic solvents make it suitable for use in both normal-phase and reversed-phase modes.
The XBridge Premier Peptide BEH C18 LC column is manufactured using Waters' proven XBridge technology, ensuring consistent column performance and reliable separation results. This column is designed to be used with Waters' Alliance 2795 Separations Module and other compatible HPLC systems. With its exceptional separation capabilities and unmatched performance, the XBridge Premier Peptide BEH C18 LC column is an essential tool for any laboratory focused on peptide research and analysis.
The Waters XBridge Premier PEPTIDE BEH C18 130 2.5 4.6 x 50 mm LC column is a high-performance reversed-phase chromatography column designed for the separation and analysis of peptides and proteins. Here are some pros and cons of buying this column:
Pros:1. High Selectivity and Efficiency: This column is known for its excellent selectivity and efficiency in separating complex peptide mixtures. It has a large pore size (130 ) and a C18 stationary phase, which allows for a wide range of interactions with peptides and proteins.
2. Wide Application Range: The XBridge Premier PEPTIDE BEH C18 column can be used for various applications such as proteomics, peptide mapping, and quality control of peptide-based drugs. It is also suitable for use with both normal-phase and reversed-phase mobile phases.
3. Compatibility with Different Detection Systems: This column is compatible with various detection systems, including UV, fluorescence, and mass spectrometry.
4. High Resolution and Sensitivity: The column provides high-resolution separations and high sensitivity, making it suitable for the analysis of trace amounts of peptides and proteins.
5. Robust and Reliable: The column is known for its robustness and reliability, with good column lifetime and consistent performance.
Cons:1. Cost: The Waters XBridge Premier PEPTIDE BEH C18 column is a high-performance and high-cost column. It may not be affordable for all laboratories or researchers.
2. Requires Specific Mobile Phases: The column may require specific mobile phases to achieve optimal performance, which may add to the cost and complexity of the analysis.
3. May Not be Suitable for All Peptides: While this column is excellent for separating many peptides, it may not be suitable for all types of peptides, particularly those with extreme hydrophobicity or hydrophilicity.
4. May Require Optimization: The column may require optimization of conditions such as pH, temperature, and mobile phase composition to achieve the best separations.
Conclusion:The Waters XBridge Premier PEPTIDE BEH C18 130 2.5 4.6 x 50 mm LC column is a high-performance column designed for the separation and analysis of peptides and proteins. It offers excellent selectivity, efficiency, and compatibility with various detection systems. However, it is a high-cost column that may not be affordable for all laboratories or researchers. It may also require specific mobile phases and optimization of conditions to achieve optimal performance. Overall, if the cost and complexity are not a limitation, this column is an excellent choice for laboratories and researchers working with complex peptide mixtures.
Recommendation:If the Waters XBridge Premier PEPTIDE BEH C18 column is not an affordable option, there are other high-performance reversed-phase columns available from different manufacturers that may offer similar or comparable performance at a lower cost. Some alternatives to consider include the Thermo Scientific Hypersil Gold C18 column, the Agilent Zorbax SB-C18 column, and the Phenomenex Jupiter Proteo C18 column. These columns have similar pore sizes and stationary phases and offer excellent selectivity and efficiency for the separation of peptides and proteins. However, they may have different application ranges or compatibility with certain detection systems, so it is important to evaluate their specifications and performance characteristics before making a decision.
Rarely used, in very good condition.