
( Brand: Thermo Scientific Electron ), ( Manufacturer Part Number: 25403-102130 ), ( Country Of Origin: United States ), ( Intended Use/discipline: Hplc Column )
The Thermo Scientific Hypersil Gold 100x 2.1mm HPLC Column, product number 25403-102130, is a high-performance reversed-phase chromatography column designed for use in liquid chromatography applications. This particular column features a 100 pore size and a 2.1 mm inner diameter, making it an excellent choice for use in ultra-performance liquid chromatography (UPLC) systems and other applications where high efficiency and small sample volumes are required.
The Hypersil Gold stationary phase consists of gold particles coated with a thin film of silica, which provides exceptional column efficiency and stability. The gold particles also offer improved mass transfer properties, resulting in faster separations and increased peak capacity.
The Hypersil Gold 100x 2.1mm column is compatible with a wide range of mobile phases, including water, acetonitrile, methanol, and various buffers. It can be used with a variety of detection systems, including UV, fluorescence, and mass spectrometry.
The 100 pore size of this column is suitable for separating small to medium molecules, such as peptides, proteins, and small organic compounds. The column's high efficiency, which is determined by the number of theoretical plates (N), is achieved through the combination of its small particle size and the optimized stationary phase.
The Hypersil Gold 100x 2.1mm HPLC column is manufactured to the highest standards of quality and consistency, ensuring excellent reproducibility from batch to batch. The column is also supplied with a stainless steel guard column to protect the analytical column from contaminants and particles that may be present in the sample or mobile phase.
The Thermo Scientific Hypersil Gold 100x 2.1mm HPLC Column is an ideal choice for researchers, quality control laboratories, and other applications where high-performance liquid chromatography is required. Its small inner diameter and high efficiency make it an excellent tool for separating and identifying complex mixtures of compounds, while its compatibility with a wide range of mobile phases and detection systems makes it a versatile and valuable addition to any laboratory.
The Thermo Scientific Hypersil Gold 100x2.1mm HPLC column (product number 25403-102130) is a popular choice for high-performance liquid chromatography (HPLC) applications due to its unique properties and high efficiency. Here are some pros and cons of buying this column:
Pros:1. High Efficiency: The Hypersil Gold column is known for its high efficiency, which means it can separate complex mixtures with excellent resolution. It has a particle size of 1.9 microns and a pore size of 100 Angstroms, which allows for rapid analysis and high resolution.
2. Long Column Life: The Hypersil Gold column has a long column life due to its gold-particle technology, which provides excellent stability and reduces column bleed. This can save time and money in the long run by reducing the need for frequent column replacements.
3. Wide Application Range: The Hypersil Gold column is suitable for a wide range of applications, including the separation of small and large molecules, polar and non-polar compounds, and acidic and basic compounds. It is commonly used in pharmaceutical, food, and environmental analysis.
4. Reputable Brand: Thermo Scientific is a reputable brand in the field of HPLC and related technologies. They offer high-quality products and excellent customer support.
Cons:1. High Price: The Hypersil Gold column is more expensive than some other HPLC columns on the market. This can be a significant consideration for laboratories or research groups with limited budgets.
2. Requires Proper Handling: The Hypersil Gold column requires proper handling and maintenance to ensure optimal performance. This includes careful conditioning before use, proper storage, and avoiding exposure to extreme temperatures or harsh chemicals.
3. Limited Capacity: The Hypersil Gold column has a limited capacity, which means it is not suitable for separating large volumes of samples. This can be a limitation for laboratories or research groups that need to analyze large sample sizes.
Ending Conclusion:The Thermo Scientific Hypersil Gold 100x2.1mm HPLC column is a high-performance column with excellent efficiency and long column life. It is suitable for a wide range of applications and offers excellent stability and reproducibility. However, it is more expensive than some other columns and requires proper handling and maintenance. Based on its unique properties and high performance, this column is recommended for laboratories or research groups that require high-resolution separation of complex mixtures and are willing to invest in a high-quality column.
Reproducible: Manufactured to ensure consistent performance column-to-column, making it a reliable choice for long-term method development. New out of Box Condition, Thermo Hypersil GOLD PFP 100x 2.1mm 3 m HPLC Column, Part No: 25403-102130, Its a specialized reversed-phase column designed for high-resolution separations. It features a PFP stationary phase, which provides unique selectivity for hallucinated compounds, isomers, and polar analyses that are often difficult to resolve on standard C18 columns. Exceptional Peak Shape: Built on high-purity silica with reduced signal activity, these columns minimize peak tailing, even for challenging basic pharmaceutical compounds.
Broad pH Stability: Suitable for a wide range of applications, demonstrating stability from 1.0 up to approximately 11.0, Enhanced Sensitivity: The symmetrical peaks produced by the Hypersil GOLD media allow better peak integration and higher sensitivity, which is critical impurity assays.