THERMO SCIENTIFIC 25705-052130 Hypersil Amino Hplc Column 50X2.1MM 5UM
( Brand: Thermo Scientific ), ( Manufacturer Part Number: 25705-052130 ), ( Country Of Origin: United States ), ( Intended Use/discipline: Hplc Column )
Review THERMO SCIENTIFIC New Hypersil Gold Amino 50x2.1mm 5um Hplc Column
The Thermo Scientific Hypersil Gold Amino 50 x 2.1 mm, 5 um HPLC Column is a high-performance chromatography column designed for the separation and analysis of amino acids and peptides. This column, with the part number 25705-052130, is manufactured by Thermo Fisher Scientific, a leading provider of analytical instruments and laboratory reagents.
The Hypersil Gold Amino column features a unique stationary phase made of gold-bonded amino functional groups. The gold bonding enhances the efficiency and selectivity of the column by increasing the interaction between the analytes and the stationary phase. The 5 um particle size offers a good balance between resolution and analysis speed, making it suitable for both routine and challenging separations.
The column dimensions are 50 mm in length and 2.1 mm in internal diameter, providing an adequate sample loading capacity and a reasonable analysis time. The column's surface area is approximately 150 m , ensuring a high number of theoretical plates and excellent separation efficiency.
The Thermo Scientific Hypersil Gold Amino column is compatible with a wide range of mobile phases, including water, acidic and basic buffers, and organic solvents. It can be used for both normal-phase and reversed-phase separations, offering flexibility for various applications.
The column is packed with a high-purity silica material and undergoes rigorous quality control tests to ensure consistent and reproducible results. The Hypersil Gold Amino column is suitable for use with most HPLC systems and autosamplers.
In conclusion, the Thermo Scientific Hypersil Gold Amino 50 x 2.1 mm, 5 um HPLC Column is a versatile and high-performance chromatography column designed for the separation and analysis of amino acids and peptides. Its unique stationary phase, gold-bonded amino functional groups, and compatibility with various mobile phases make it an excellent choice for both routine and challenging separations.
The Thermo Scientific Hypersil Gold Amino 50x2.1mm, 5um HPLC column is a popular choice for protein and peptide separations due to its unique stationary phase composition and particle size. Here are some pros and cons to consider before making a purchase.
Pros:1. Excellent separation efficiency: The 5um particle size and the unique Gold bonded amino phase provide excellent separation efficiency for proteins and peptides, making it a popular choice for proteomics and peptide mapping applications.
2. High loading capacity: The column can handle high sample loads, which is essential for complex protein mixtures.
3. Good resolution and reproducibility: The column offers good resolution and reproducibility, which is critical for accurate and reliable analysis.
4. Compatible with various mobile phases: The column can be used with various mobile phases, including acidic and basic mobile phases, making it a versatile option for a wide range of applications.
Cons:1. Limited column length: The column length is relatively short, which may limit its ability to separate larger proteins or complex protein mixtures.
2. Higher cost: Compared to other HPLC columns, the Thermo Scientific Hypersil Gold Amino column is more expensive.
3. Requires careful handling: The column requires careful handling and maintenance to ensure its longevity and optimal performance.
In conclusion, the Thermo Scientific Hypersil Gold Amino 50x2.1mm, 5um HPLC column is a high-performance column for protein and peptide separations, with excellent separation efficiency, high loading capacity, good resolution, and versatility in mobile phases. However, it has a limited column length, higher cost, and requires careful handling.
Based on the pros and cons, it is recommended for researchers and laboratories that require high separation efficiency and good resolution for protein and peptide separations, particularly for proteomics and peptide mapping applications, where accuracy and reliability are essential. If your application does not require the high separation efficiency or resolution offered by this column, it may be worth considering other, less expensive options.
Details:
Versatile Modes: Ideal for carbohydrate analysis normal phase and the separation of polar compounds. It features a High-Performance Improperly Phase on Ultra pure Silica, making it suitable for weak Anion Exchange, Reversed-Phase, and normal-Phase Modes. New out of Box Condition, Thermo Scientific Hypersil GOLD Amino 50x2.1mm 5um HPLC Column, Part No: 25705-052130, Its a High-Performance Analytical Column designed for Versatile Chromatography Separations. Peak Shape: The ultra pure silica base reduces secondary interactions, leading to improved peak symmetry and sensitivity compared older silica-based columns.
HILIC Compatibility: Can be used in Hydrophilic Interaction Liquid Chromatography HILIC mode.
part #: 25705052130 price
- $745.00
specifications labequipi:
- brand: Thermo Scientific
- mpn: 25705-052130
- country of origin: United States
- intended use/discipline: Hplc Column
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- condition: New Open box
- Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment
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