
( Brand: Thermo Scientific ), ( Manufacturer Part Number: 5300170 )
The Thermo Scientific Luminescent Plate Reader Luminometer Ascent Microplate Reader Model 5300170, produced by Thermo Fisher Scientific, is a versatile and advanced instrument designed for the quantitative measurement of luminescent and fluorescent signals in various assay formats. This luminometer is a part of the Luminoskan Ascent family, which is renowned for its high-performance and user-friendly design.
The Ascent Microplate Reader offers a wide dynamic range, enabling it to accurately measure a broad range of luminescent signals, from weak to strong. It is capable of detecting signals as low as 10 5 relative light units (RLUs) and as high as 10 15 RLUs. This exceptional sensitivity makes it an ideal instrument for various applications, including cell viability assays, luciferase assays, and homogeneous and heterogeneous enzyme assays.
The reader features an advanced optics system, including high-performance filter sets, which ensures precise and accurate detection of luminescent and fluorescent signals. Its dual-mode capability allows for the simultaneous measurement of both luminescence and fluorescence in the same well, making it a valuable tool for multifunctional assays.
The Ascent Microplate Reader is designed with an intuitive, user-friendly interface that allows for easy operation and data analysis. Its software, WinLuminescence, offers a range of features, including well-by-well analysis, standard curve fitting, and real-time data display. Additionally, the reader is compatible with various microplate formats, including 96-, 384-, and 1536-well plates.
The instrument boasts a robust construction, ensuring durability and reliability in various laboratory environments. It features a compact design, which saves valuable bench space, and a quiet operation, making it a suitable addition to any laboratory. The reader also offers a high degree of flexibility, with the ability to customize detection settings and automate data analysis.
In summary, the Thermo Scientific Luminescent Plate Reader Luminometer Ascent Microplate Reader Model 5300170 is a versatile, high-performance, and user-friendly instrument designed for the accurate measurement of luminescent and fluorescent signals in various assay formats. Its advanced features, exceptional sensitivity, and user-friendly interface make it an indispensable tool for researchers and laboratory professionals in various fields.
The Thermo Scientific Luminescent Plate Reader Luminoskan Ascent Microplate Luminometer 392 is a popular choice for laboratories requiring high-performance and accurate luminometry measurements. Here are some pros and cons of buying this instrument:
Pros:1. High Sensitivity: The Luminoskan Ascent Microplate Luminometer 392 offers a high level of sensitivity, making it suitable for various assays including luciferase activity, ATP measurement, and protein-protein interactions.
2. Versatility: This instrument can be used for both single-wavelength and multi-wavelength measurements, making it a versatile tool for different applications.
3. Large Sample Capacity: The Luminoskan Ascent Microplate Luminometer 392 can read up to 384-well microplates, allowing for large sample throughput.
4. Advanced Software: Thermo Fisher Scientific's software, Luminescence 2.20, offers a user-friendly interface, data analysis capabilities, and the ability to customize experimental parameters.
5. Reliable: The instrument is known for its robustness and reliability, with minimal maintenance requirements and a long lifespan.
Cons:1. Expensive: The Thermo Scientific Luminoskan Ascent Microplate Luminometer 392 is a high-end instrument, and its price reflects its advanced features and capabilities.
2. Limited Wavelength Range: Although it can be used for multi-wavelength measurements, the instrument's wavelength range is not as extensive as some other luminometers on the market.
3. Large Footprint: The instrument requires a substantial amount of bench space due to its size, which can be a disadvantage for laboratories with limited space.
4. Requires Calibration: Regular calibration is necessary to maintain accurate and precise measurements, which can add to the cost and time required for operation.
Conclusion:The Thermo Scientific Luminoskan Ascent Microplate Luminometer 392 is a reliable and versatile instrument with high sensitivity and advanced software capabilities. Its ability to read large sample volumes and perform both single-wavelength and multi-wavelength measurements makes it an excellent choice for laboratories requiring accurate luminometry measurements. However, its high cost, limited wavelength range, and large footprint may be disadvantages for some laboratories. Ultimately, the decision to purchase this instrument should be based on the specific requirements and budget of the laboratory.
Recommendation:If your laboratory requires high-performance and accurate luminometry measurements, the Thermo Scientific Luminoskan Ascent Microplate Luminometer 392 is a strong consideration. Its advanced features and capabilities make it an excellent investment for laboratories with the necessary budget and space requirements. However, if your laboratory has limited resources, you may want to consider alternative, more affordable luminometers with similar capabilities.
No software for this one so no further testing done. The tray moves in and out by pressing the button inside front cover. Used but in clean condition as shown the pictures. I installed the transport lock for safe shipping.
Item# 225201L28A. Powers on and the front LED goes to solid green after startup.