
( Brand: Agilent ), ( Manufacturer Part Number: 79916AP-574 ), ( Part Type: Cartridge )
The Agilent 79916AP-574 APS Hypersil T1 Column is a high-performance reversed-phase HPLC (High-Performance Liquid Chromatography) analytical column designed for use with aqueous mobile phases. This particular column, part of the Agilent APS Hypersil TM series, is ideal for separating and analyzing small to medium sized molecules, particularly those with a hydrophobic character.
The packing material for this column is made up of sub-2 micron, spherical, porous silica particles. The particles have a pore size of 100 , ensuring that they offer a large surface area for efficient interactions with the analytes. Additionally, the small particle size enables increased efficiency and faster separation times. The stationary phase of the column is coated with a hydrophilic end capping, which aids in reducing column bleed and improving column efficiency.
The column measures 75 mm in length and 4.6 mm in diameter, making it a standard size for most HPLC systems. The total pore volume of the column is 1.8 mL, with a theoretical plate count of approximately 11,000 plates. This high plate count results in excellent separation efficiency and the ability to resolve complex mixtures.
The Agilent 79916AP-574 APS Hypersil T1 Column is specifically designed for use with aqueous mobile phases, and it is particularly well-suited for separating acidic or basic compounds. The column is stable under a wide range of pH conditions, from pH 2 to pH 10, and it can be used with a variety of mobile phases, including water, acetonitrile, methanol, and acids such as formic or trifluoroacetic acid.
This column is also compatible with various HPLC detection techniques, such as UV-Vis, fluorescence, and mass spectrometry (MS), making it a versatile tool for a wide range of applications in fields such as pharmaceuticals, biotechnology, and environmental analysis.
In conclusion, the Agilent 79916AP-574 APS Hypersil T1 Column is a high-performance, sub-2 micron HPLC analytical column designed for use with aqueous mobile phases. Its small particle size, large surface area, and high plate count make it an excellent tool for separating and analyzing small to medium sized hydrophobic molecules. Its compatibility with a wide range of pH conditions and detection techniques makes it a versatile and valuable addition to any HPLC laboratory.
The Agilent 79916AP-574 APS Hypersil T1 column (t10707) is a high-performance reversed-phase HPLC column designed for the separation of small to medium-sized molecules, particularly in the pharmaceutical and biotech industries. Let's discuss some pros and cons of investing in this particular column.
Pros:1. High Efficiency: This column offers excellent efficiency with up to 15,000 theoretical plates, which results in better resolution and separation of complex mixtures.
2. Broad Application Range: It is suitable for a wide range of applications, including the separation of peptides, proteins, and small organic compounds.
3. High Loading Capacity: The T1 column can handle high sample loads, making it an ideal choice for high-throughput applications.
4. Consistent Performance: This column delivers consistent and reliable results, ensuring high-quality data for regulated industries.
5. Short Analysis Time: With its high efficiency and fast stationary phase, the T1 column reduces analysis time significantly.
Cons:1. Cost: The price point for this high-performance column is relatively higher compared to standard HPLC columns.
2. Requires Careful Handling: Due to its high efficiency, the T1 column is more susceptible to contamination and requires more stringent handling conditions.
3. Limited Linert Length: The T1 column has a limited linear length of 10 cm, which may not be suitable for long separation processes.
4. Incompatible with Some Mobile Phases: This column may not be suitable for use with certain mobile phases, such as aqueous buffers with high ionic strength or strong acids.
In conclusion, the Agilent 79916AP-574 APS Hypersil T1 column (t10707) offers significant advantages such as high efficiency, broad application range, high loading capacity, consistent performance, and short analysis time. However, its higher cost, susceptibility to contamination, limited linear length, and incompatibility with certain mobile phases should be carefully considered before making a purchase.
Based on the information provided, it is recommended that this column be considered for applications where high efficiency, fast separation, and reliable results are essential, particularly in the pharmaceutical and biotech industries. However, it may not be the best choice for applications with limited budgets or where longer separation times are acceptable.
Classification: Item is used, sold as pictured, 30 Day Dead on Arrival DOA Warranty. Glad you stopped by. Open to Making Bulk Deals. Extra Information:.
Specs: 200 X 4.6MM 5UM. We proudly sell hard-to-find parts, components, and machines to makers manufacturers. Physical condition: Very Good Item looks excellent. AGILENT 79916AP-574 APS HYPERSIL HPLC CARTRIDGE T10707 Brand:: AGILENT, condition: This is a used item, in good condition and includes 30 day warranty.
Alternative Part/ model Number: S/N 388. Rush Orders Ship Same Day.