AGILENT 799775-944 Advancebio Mab Diphenyl 2.1 50 3.5MICRON

( Brand: Agilent ), ( Manufacturer Part Number: 799775-944 ), ( Unit Type: Lb ), ( Country Of Origin: United States ), ( Intended Use/discipline: Biological Laboratory, Immunology, Medical Laboratory, Physical Laboratory ), ( California Prop 65 Warning: California Prop 65 Applicable )

Review AGILENT Advancebio Rp mab Diphenyl 2.1 50mm 3.5micron

The Agilent 799775-944 Advanced BioRP-MAB Diphenyl 2.1 x 50 mm, 3.5 m HPLC Column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of biomolecules, particularly proteins and peptides, in complex biological matrices.

This column is part of Agilent's Advanced BioRP (Reversed-Phase) product line, which is known for its exceptional selectivity and efficiency in separating biomolecules. The MAB (Monotypic Anionic Bonded) stationary phase is based on a diphenyl structure that provides excellent hydrophobic interaction, making it ideal for the separation of hydrophobic proteins and peptides.

The column has a total length of 50 mm and an internal diameter of 2.1 mm, making it suitable for use in standard HPLC systems. The particle size of the stationary phase is 3.5 m, which ensures high resolution and rapid separation of analytes. The column is packed with 5 m guard beads to protect the analytical column from contamination and particle clogging.

The Agilent 799775-944 column is specifically designed for use with aqueous mobile phases and can operate over a wide pH range (1.8-7.5). It is compatible with various detection systems, including UV, fluorescence, and mass spectrometry. The column also features a low backpressure and high loading capacity, allowing for high sample throughput and increased productivity.

The stationary phase of this column is bonded with a monotypic anionic bonding agent, which provides excellent retention and selectivity for basic and acidic proteins and peptides. The column also exhibits excellent reproducibility and stability, ensuring consistent results over multiple analyses.

Overall, the Agilent 799775-944 Advanced BioRP-MAB Diphenyl 2.1 x 50 mm, 3.5 m HPLC Column is a versatile and powerful tool for separating and analyzing complex biological samples, making it an essential component for researchers and laboratories in the fields of proteomics, peptide analysis, and biopharmaceuticals.

Agilent 799775-944 AdvanceBio RP-MAB Diphenyl 2.1 x 50 mm, 3.5 m Column: A Detailed Analysis of Its Pros and Cons

The Agilent 799775-944 AdvanceBio RP-MAB Diphenyl 2.1 x 50 mm, 3.5 m column is a popular choice for protein separation and analysis in liquid chromatography (LC). In this analysis, we will discuss the key benefits (pros) and potential drawbacks (cons) of investing in this particular LC column.

1. High-resolution separations: The 3.5 m particle size allows for increased efficiency and higher resolution separations, enabling the identification and quantification of closely eluting proteins.

2. Broad applicability: The Diphenyl stationary phase is suitable for a wide range of proteins and peptides, making it a versatile choice for various research applications.

3. Improved sensitivity: The small particle size and optimized stationary phase enhance the sensitivity of the LC system, allowing for the detection of lower-abundant proteins and peptides.

4. Durable and robust: The AdvanceBio RP-MAB column is known for its robustness, withstanding harsh mobile phase conditions and providing stable and consistent peak shapes over extended periods.

5. Easy-to-use: The column is compatible with most LC systems and automatic sample preparation instruments, ensuring easy integration into existing workflows.

1. Higher cost: Compared to standard LC columns, the Agilent 799775-944 AdvanceBio RP-MAB Diphenyl 2.1 x 50 mm, 3.5 m column comes with a higher price tag due to its advanced features and superior performance.

2. Requires specialized knowledge: Proper use and optimization of the column may require specialized knowledge and expertise in protein separation techniques, which could be a barrier for some researchers.

3. Limited sample loading capacity: The smaller particle size and increased resolution may limit the sample loading capacity, requiring smaller injection volumes and potentially increasing the overall analysis time.

The Agilent 799775-944 AdvanceBio RP-MAB Diphenyl 2.1 x 50 mm, 3.5 m column offers several advantages over standard LC columns, including improved sensitivity, high-resolution separations, and broad applicability. However, its higher cost and potential increased complexity may make it a less attractive option for researchers with limited budgets or expertise. Ultimately, the decision to invest in this column depends on the specific research needs and resources available.

If your research involves the analysis of complex protein mixtures, the identification and quantification of closely eluting proteins, or the need for increased sensitivity, the Agilent 799775-944 AdvanceBio RP-MAB Diphenyl 2.1 x 50 mm, 3.5 m column could be an excellent investment. However, if budget constraints or a lack of specialized knowledge are concerns, it may be worth considering alternative, more cost-effective LC columns for your research.

Details:

Perfect for researchers and scientists seeking reliable efficient separation analysis of their samples.

part #: 799775944 prices

• $399.00-$569.00

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• brand: Agilent
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