
( Brand: Invitrogen ), ( Manufacturer Part Number: 8003000 ), ( Part Type: Tube ), ( Country/region Of Manufacture: United States ), ( Intended Use/discipline: Biological Laboratory, Medical Laboratory, Microbiological Laboratory )
The Invitrogen PureLink PCR Spin Columns are a high-performance tool designed for the purification and cleaning of PCR products in a quick and efficient manner. These columns, sold in packs of 50, are suitable for use with various PCR applications, including standard, long range, and amplification of RNA and DNA.
The columns utilize a silica-membrane based technology, which effectively binds and retains the PCR amplicons while allowing the elution of unbound substances, including unincorporated primers and dNTPs, salts, and enzyme inhibitors, resulting in a highly pure and elegant DNA or RNA product. This technology also ensures minimal shearing and no loss of size or yield, preserving the integrity of your valuable samples.
The Invitrogen PureLink PCR Spin Columns offer a simple, one-step purification process with a short processing time. The columns can be easily used in any laboratory setting, as they do not require any specialized equipment or extensive training. Each column is pre-satisfied with binding buffer, ensuring ease of use and consistent results.
The columns are compatible with a wide range of reaction volumes, from less than 10 L to over 200 L. They are also columns can be used with both standard and high-salt buffers, accommodating various PCR conditions. The flow-through design of the spin columns ensures that there is no need for vacuum manipulation or multiple spin steps, making the purification process quick and hassle-free.
The 50-pack of Invitrogen PureLink PCR Spin Columns is an excellent choice for laboratories that frequently perform PCR purifications or have a high volume of samples to process. These columns provide consistent, reliable results, ensuring that your downstream applications achieve optimal performance. The high capacity and ease of use make these columns a versatile and cost-effective solution for various PCR applications.
The Invitrogen PureLink PCR Spin Columns are delivered in a convenient and space-saving tray, allowing you to store and use the columns right out of the box. These columns are guaranteed to provide you with a highly purified PCR product, ensuring your downstream applications achieve optimal results.
1. High Capacity: Each column can process up to 200 L of sample, making it suitable for large-scale PCR reactions or multiple samples.
2. Easy to Use: The spin column design allows for quick and easy DNA purification, reducing the time and effort required for downstream applications.
3. Robust: These columns have been designed for routine usage and can handle different types of PCR reactions including high GC, GC-rich, and complex genomic samples.
4. Versatile: The columns can be used for various downstream applications, such as cloning, sequencing, library preparation, and quantification.
5. Thorough Clean-up: The columns effectively remove larger contaminants, unincorporated primers, and excess dNTPs, ensuring high-quality PCR products.
6. Eluted Sample Holds up to 200 L: The ability to elute a larger volume increases the versatility of these columns in various PCR applications.
7. Great for Large-scale Research: In terms of cost-effectiveness, buying a pack of 50 columns can save research laboratories a substantial amount of money in the long run.
Cons:1. Requires Additional Consumables: The PCR purification process involves more consumables, such as PCR reaction buffers and nucleotides, which may add to the overall cost.
2. Fabric Filter: There is a risk of clogging the fabric filter with PCR fragments or residual contaminants during the spinning step, which may affect the functionality of the column. However, this can be minimized by careful handling.
Conclusion:The Invitrogen PureLink PCR Spin Columns provide a simple and efficient solution for purifying PCR products in large-scale research environments. Though they come with some additional consumables and require careful handling, their versatility, ease-of-use, and high capacity outweigh these drawbacks. The cost-effective purchase of a pack of 50 columns recommends these as a formidable choice for laboratories involved in large-scale PCR reactions or processing numerous samples.
VWR Catalog #: K310001 for 50 Preps - PureLink PCR Purification Kit. Condition: New, Exp: 2024-09-30. VWR Catalog #: K310002 for 50 Preps - PureLink PCR Purification Kit. The Sedna binds to the silica-based membrane in column.
Advantages of the PureLink PCR Purification Kit include. Specifications. Invitrogen 8003000 Pure Link PCR Spin Columns with Collection Tubes, Pack of 50. The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Remove impurities by thorough washing with Wash Buffer. Simply mix the PCR product with Binding Buffer and apply to PureLink Spin Column. The PureLink PCR Purification Kit is based on the selective binding of Sedna to silica-based membrane in presence tropic salts.