
( Brand: Chemometec ), ( Manufacturer Part Number: 900-0004 ), ( Part Type: Cell ), ( Unit Type: Unit ), ( Country/region Of Manufacture: United States ), ( Intended Use/discipline: Biological Laboratory )
The Chemometec NucleoCounter NC-3000 Mammalian Cells Power Kit (product code: 900-0004) is a versatile and essential tool for accurate and efficient cell counting and particle analysis in various research fields, particularly in the field of mammalian cell science. This kit includes the essential reagents required for using the NucleoCounter NC-3000 system to determine cell viability and nucleic acid content in suspensions of mammalian cells.
The kit consists of two main components: the CyStain UV precise P1 reagent and the CyStain Lysing Solution. The CyStain UV precise P1 reagent is a nucleic acid staining solution that selectively stains nucleic acids in cells, allowing for discrimination between viable and dead cells based on their nucleic acid content. This reagent contains a low background fluorochrome, ensuring high sensitivity and specificity.
The CyStain Lysing Solution is a lysing agent designed to gently lyse the cell membranes, releasing the cellular contents for accurate measurement by the NucleoCounter NC-3000 system. This solution effectively maintains the integrity of the cellular components, ensuring accurate and reliable results.
The NucleoCounter NC-3000 system utilizes Coulter principle technology, which measures the electrical impedance caused by the passage of individual cells through a small aperture. This technology allows for the detection and analysis of cells based on their size, complexity, and nucleic acid content, providing valuable information on cell viability, population heterogeneity, and cell cycle analysis.
The Chemometec NucleoCounter NC-3000 Mammalian Cells Power Kit (product code: 900-0004) is an indispensable tool for researchers working with mammalian cells, offering high accuracy, ease of use, and reproducibility. Its ability to provide real-time, label-free analysis of cell viability and nucleic acid content makes it an invaluable resource for various applications, including cell culture monitoring, drug discovery and development, toxicology studies, and gene therapy research.
The Chemometec 900-0004 NucleoCounter Mammalian Cells Power is an automated cell counter designed for the precise and accurate determination of nucleated cell counts and viability in various types of samples, including mammalian cells. Here are some pros and cons of buying this instrument:
Pros:1. High Accuracy: The NucleoCounter uses a flow cytometric method for counting cells, which provides high accuracy and reliability. It can distinguish between dead and live cells, as well as different types of cells based on their size and DNA content.
2. Easy to Use: The instrument is user-friendly and requires minimal sample preparation. Samples can be loaded directly into the instrument without the need for centrifugation or staining.
3. Fast Analysis: The NucleoCounter can analyze up to 5,000 events per second, providing rapid results within minutes.
4. Versatile: The instrument can be used for a wide range of applications, including cell culture, drug discovery, toxicology, and clinical diagnostics.
5. Low Sample Volume: The instrument requires only 10-50 L of sample per analysis, making it an efficient option for working with limited sample volumes.
Cons:1. High Cost: The NucleoCounter is a high-end instrument and comes with a significant price tag. It may not be an affordable option for laboratories with limited budgets.
2. Requires Regular Maintenance: The instrument requires regular maintenance and calibration to ensure accurate and reliable results. This can add to the cost and time required to use the instrument.
3. Complexity: The instrument may be more complex than manual cell counting methods, requiring specialized training and expertise to operate and maintain.
4. Limited Sample Types: The instrument is designed for mammalian cells and may not be suitable for counting other types of cells, such as bacteria or yeast.
In conclusion, the Chemometec 900-0004 NucleoCounter Mammalian Cells Power is a high-performance instrument that offers accurate and rapid cell counting and viability analysis for mammalian cells. Its ease of use, versatility, and fast analysis make it a valuable tool for laboratories in various fields. However, its high cost, complexity, and limited compatibility with sample types other than mammalian cells are potential drawbacks to consider.
If your laboratory requires accurate and reliable cell counting and viability analysis for mammalian cells, and you have the budget and expertise to operate and maintain a high-end instrument, then the Chemometec 900-0004 NucleoCounter Mammalian Cells Power may be a worthwhile investment. Alternatively, if your laboratory has limited resources, you may consider manual cell counting methods or less expensive alternatives. Ultimately, the decision to purchase the instrument should be based on your specific research needs and budget considerations.
It is being sold AS-IS and WITHOUT WARRANTY. Shipping Buyer pays for all shipping costs. Orders are shipped to the cardholders billing address. Warranty This item is guaranteed to be as described.
CHEMOMETEC NUCLEOCOUNTER 900-0004 MAMMALIAN CELLS - DOES NOT POWER: This lot is for one Chemometec NucleoCounter. Power cords and power adapters are never included unless specifically mentioned. Does not power on when attempted. This unit will arrive in the condition shown.