
( Brand: Thermo Fisher ), ( Manufacturer Part Number: A28135 )
The Thermo Fisher Scientific A28135 QuantStudio 3D Digital PCR System from Applied Biosystems is a state-of-the-art digital PCR (dPCR) system designed for high-throughput quantitative polymerase chain reaction (qPCR) experiments. This advanced system enables precise and absolute quantification of nucleic acid targets, providing accurate and reliable results even in complex samples.
The QuantStudio 3D Digital PCR Chip, which is specifically designed for use with this system, features a 384-well format that allows for increased throughput and efficiency in large-scale experiments. Each well on the chip has a unique partitioning barrier that physically separates the reaction volumes during PCR, resulting in precise and independent amplification of target nucleic acids.
The A28135 system utilizes the proven QuantStudio Design and Analysis Software for data acquisition and analysis. This intuitive software allows for easy experiment setup, real-time data visualization, and comprehensive reporting. It also includes built-in algorithms for automated clustering and droplet identification, ensuring accurate and consistent results.
The system is equipped with several advanced features, including automatic droplet identification and signal processing, which help to minimize user intervention and increase throughput. It also includes an integrated thermal control system that enables temperature ramping rates up to 4 C per second, ensuring rapid and efficient PCR cycles.
The Thermo Fisher Scientific A28135 QuantStudio 3D Digital PCR System is ideal for a wide range of applications, including gene expression analysis, mutation detection, and absolute quantification of specific nucleic acid targets. Its high-throughput capabilities and precise, accurate results make it a valuable tool for research laboratories, diagnostic facilities, and other applications where quantitative analysis of nucleic acids is essential.
Overall, the Thermo Fisher Scientific A28135 QuantStudio 3D Digital PCR System is a powerful and versatile instrument that offers significant advantages over traditional qPCR methods for nucleic acid quantification. Its advanced features, high-throughput capabilities, and ease of use make it an indispensable tool for researchers and laboratory professionals in a variety of fields.
The Thermo Fisher Scientific A28135 Applied Biosystems QuantStudio QS5 Real-Time PCR System is a versatile and advanced instrument designed for quantitative polymerase chain reaction (qPCR) applications. This system offers several advantages and disadvantages that should be considered before making a purchase decision.
2. Pros:a. High-throughput capabilities: The QuantStudio QS5 can process 384-well plates, enabling users to perform large-scale qPCR experiments in a single run.
b. Improved sensitivity: This system utilizes advanced detection technology, allowing for increased sensitivity and accurate quantification of target genes.
c. User-friendly interface: The instrument features an intuitive touchscreen interface, making it easy for researchers to design experiments, analyze data, and optimize reaction conditions.
d. Flexible thermal cycling program: The QuantStudio QS5 offers various thermal cycling programs, enabling users to customize their experiments to suit their specific research needs.
e. Integrated data analysis software: The system comes with software that can be used for data analysis, including Ct value calculation, melting curve analysis, and real-time plotting.
3. Cons:a. High cost: The QuantStudio QS5 is a high-end PCR system and comes with a significant investment. The prices may be prohibitive for some research groups.
b. Requires regular maintenance: To maintain optimal performance, the instrument requires routine maintenance and calibration, which can be time-consuming and costly.
c. Limited sample volume: Although the system can handle 384-well plates, the total sample volume per well is relatively low compared to other systems, which may not be suitable for some applications.
4. Conclusion:The Thermo Fisher Scientific A28135 Applied Biosystems QuantStudio QS5 Real-Time PCR System offers several advantages, such as high-throughput capabilities, improved sensitivity, user-friendly interface, flexible thermal cycling programs, and integrated data analysis software. However, it also comes with some disadvantages, including a high cost, requirement for regular maintenance, and limited sample volume per well.
5. Recommendation:If your research group requires a high-throughput, sensitive, and user-friendly qPCR system, the Thermo Fisher Scientific A28135 Applied Biosystems QuantStudio QS5 Real-Time PCR System could be an excellent investment. However, it is essential to consider the investment cost, maintenance requirements, and sample volume constraints before making a final decision. Researchers should carefully evaluate their research needs and budget to determine if the benefits of this system outweigh the costs.
Thermo Applied Biosystems QuantStudio 5 QS5 384-well real-time qPCR system: Great working used condition. If you require expedited shipping or wish to be billed your FedEx UPS DHL account# please let us know upon purchase. 2025 Blabbed. Requires a mouse for screen control, not sure if touch is working or just setting function off.
Includes a power cable, WiFi dongle. 14,170 cycle count.