THERMO SCIENTIFIC A80400112 Module Immunohistochemistry Pretreatment

( Brand: Thermo Scientific ), ( Manufacturer Part Number: A80400112 ), ( Part Type: Module )

Review THERMO SCIENTIFIC Pt Module Immunohistochemistry Pretreatment Parts

The Thermo Scientific A80400112 Module for Immunohistochemistry (IHC) Pretreatment is a versatile and essential tool for enhancing the detection and visualization of antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections. This module is specifically designed for use with the Ventana Discovery XT and Ventana BenchMark ULtra IHC staining systems, ensuring optimal performance and consistency in your research or diagnostic applications.

The A80400112 module includes several reagents and buffers that facilitate antigen retrieval and epitope unmasking, critical steps in the IHC process. These reagents are formulated to be compatible with a wide range of primary antibodies and detection systems, making this module an excellent choice for various research and diagnostic applications.

The module begins with the CC1 buffer, which is designed for heat-induced epitope retrieval. This buffer contains citrate, a weak acid that helps to dissolve the calcium-based cross-links in the paraffin-embedded tissue sections. The retrieval process is carried out in a pressure cooker or a steamer, and the optimal temperature and time depend on the specific antigen being targeted.

Following epitope retrieval, the slides are rinsed with the supplied Tris-buffered saline (TBS), pH 8.0, to neutralize the acidic buffer and prepare the slides for further processing. The next step is the application of the enzyme solution, which contains proteases, such as trypsin or protease III, that degrade the complex protein structures in the tissue sections, exposing the hidden epitopes. The enzyme solution is typically applied for 5 to 30 minutes, depending on the antigen and the chosen enzyme.

Once the enzyme solution is removed, the slides are rinsed again with TBS and then with water to remove any remaining enzyme and debris. The slides are then ready for the application of the primary antibody and the subsequent detection system.

In summary, the Thermo Scientific A80400112 Module for Immunohistochemistry Pretreatment is an essential tool for enhancing antigen detection and visualization in FFPE tissue sections. The module includes reagents and buffers for heat-induced epitope retrieval, enzyme digestion, and rinsing, ensuring optimal performance and consistent results with various primary antibodies and detection systems.

Thermo Scientific A80400112 Module Immunohistochemistry Pretreatment: A Comprehensive Analysis

The Thermo Scientific A80400112 Module Immunohistochemistry (IHC) pretreatment is a widely used solution for enhancing antigen retrieval in IHC experiments. This analysis aims to provide a balanced assessment of the advantages and disadvantages of utilizing this product.

1. Consistent results: The Thermo Scientific A80400112 Module provides reliable and consistent antigen retrieval, which is crucial for accurate IHC staining.

2. Versatility: This pretreatment module can be used with various IHC detection systems and antibodies, expanding its applicability across research fields.

3. User-friendly: The product is easy to use and requires minimal hands-on time, making it a convenient choice for laboratories.

4. Reduced background staining: The Thermo Scientific A80400112 Module effectively reduces background staining, which can enhance the visibility and specificity of the staining results.

1. Cost: The Thermo Scientific A80400112 Module is relatively expensive compared to some alternative pretreatment methods.

2. Limited compatibility: Although the product is versatile, it may not be suitable for certain types of antigens or tissue samples that require specific pretreatment conditions.

3. Potential damage to antigens: Over-exposure to the pretreatment solution can lead to antigen damage, which may impact the staining results.

The Thermo Scientific A80400112 Module Immunohistochemistry pretreatment offers several advantages, such as consistent results, versatility, user-friendliness, and reduced background staining. However, it also comes with disadvantages, including cost, limited compatibility, and potential antigen damage. Ultimately, the decision to use this product should be based on the specific requirements of the research project, available resources, and the alternatives available.

If your research project demands high-quality, consistent, and specific IHC staining results, and you have the necessary budget, the Thermo Scientific A80400112 Module Immunohistochemistry pretreatment is a recommended option. It offers valuable benefits that can significantly improve the IHC staining process. However, if cost is a major concern, it may be worth exploring alternative pretreatment methods that can provide similar results at a lower cost.

Details:

Untitled Document.

part #: a80400112 prices

• $700.00-$1000.00

specifications labequipi:

• brand: Thermo Scientific
• mpn: A80400112
• part type: Module

seller labequipi:

• rating: 99.1%
• # reviews: 4960
• city: San Diego, California

offer labequipi:

• quantity: 1
• availability: In Stock
• options: Ship-to-home
• started: March 26, 2025
• sold: 0
• best offers: True

payments labequipi:

• other
• credit card: Mastercard
• wallet: Paypal

general labequipi:

• condition: For parts or not working
• Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment

returns labequipi:

• policy: No Returns

shipping labequipi:

• transit: 3-8 Days
• carrier: UPS
• type: Calculated
• service: UPS Ground
• class: Standard
• cost: $41.25

Thermo Scientific A80400112 Is Similar To:

• 001401-050 60053-64141 Thermo Scientific New Chromquest Chromatography Data Software Spectrasystem (36.2% similar) 2THERMO part no: 050AGILENT brand new in original package, sealed. Inc UP1A1019 chromatography data software spectra system model: remixed V4...(December 30th, 2018)
• 0028297 Thermo Scientific Electron Hatr (46.6% similar) Ships to the us only...(August 10th, 2018)
• 0028299 Thermo Scientific Electron Smart Hatr (45.9% similar) Used Thermo electron smart multi-bounce Hart for liquids and solids Scientific Nicole X700 winner to pay shipping cost of 15...(August 9th, 2018)
• 0030-091 Thermo Scientific Collector Ii Diffuse Reflectance Drifts Accessory (39.2% similar) If you don't receive a response, there is problem. This is used medical equipment. For very large items, this fee also covers some of the time and labor necessary to build a custom box or pallet. This covers the cost of box and packaging materials. For more and larger pictures spectra-tech Thermo ...(April 24th, 2018)
• 00950-10002 Thermo Scientific New Lamp Vuv Syagen Replace For Ltq Orbitrap (37.1% similar) New Thermo lamp UV Sagan replace for Lt orbit rap veils executive conditioner Scientific replacement no: note: dear valued buyer thanks doing business with us. Info: the shipping price is only for us 48 contiguous states...(December 3rd, 2018)
• 0114447 Fisher Scientific Thermo Haake Works (38% similar) Thermo fisher Scientific hake PC200 115V works overall nice condition. Some minor scratches. Unit powers on when plugged in and is fully functional, but runs a little loud. Please view pictures as part of the. Sold without power cable or other accessories...(May 17th, 2018)
• 013016md Thermo Scientific Orion Two Electrode Conductivity Cell (38.3% similar) The unit is used and shows some signs of regular wear. Are not mentioned, they included. Only what is pictured included in this listing. If manuals, software, packaging etc. We ask that you please first if would like to return a purchase...(June 10th, 2018)
• 0296140 Thermo Scientific Heating Element (42.4% similar) Document-connect. Type y heating element for Thermo fisher water stills fits with model number A1013 1000 watts 120 or 240 v service see barn stead classic stills manual. Fs-assets led manuals D01311. URL s: assets...(March 17th, 2019)
• 042151 Thermo Scientific New Prod Col Op Pn (40% similar) Make an offer. Return if not of use. New Thermo PROD,COL,OP,PAX-100G,4X50MM...(July 20th, 2018)
• 049457 Thermo Scientific O-ring Kit (39.3% similar) Thermo o-ring kit for arse units package pn: make an offer see pictures details return if not of use...(January 10th, 2018)
• 061687 Thermo Scientific Kit Seal (40% similar) Thermo kit seal cell,cap,peek,pkg of 50 for arse pn: see pictures details make an offer return if not use...(January 10th, 2018)