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THERMO SCIENTIFIC ES75750PN Spray Pepmap Rslc Neo Uhplc C18 2UM 100A Column

Es75750pn Thermo Scientific Easy-spray Pepmap Rslc C18 2um 100a Neo Uhplc Column

( Brand: Thermo Scientific ), ( Manufacturer Part Number: ES75750PN ), ( Part Type: Spray ), ( Intended Use/discipline: Easy-spray Column )

Review THERMO SCIENTIFIC Easy spray Pepmap Rslc C18 2um 100a Neo Uhplc Column

The Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC Column is a high-performance liquid chromatography (HPLC) column designed for reversed-phase separation of peptides and proteins. This particular column is part of the Easy-Spray series, which is known for its compatibility with various mass spectrometry (MS) techniques, especially those using the Easy-Spray ionization source.

The column features a C18 stationary phase with a particle size of 2.1 microns and a pore size of 100 . The smaller particle size increases the efficiency and resolution of the separations, while the larger pore size enhances the column's ability to accommodate larger molecules, such as proteins. The stationary phase has also been modified with a NeoCore surface technology, which improves the chromatographic performance and the compatibility with MS.

The Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC Column is specifically designed for use with ultrahigh-performance liquid chromatography (UHPLC) systems, allowing for faster separations and higher throughput. The column measures 150 mm in length and 2.1 mm in inner diameter, providing an adequate separation length for most applications.

The column's compatibility with various MS techniques is a significant advantage, as it allows for direct MS analysis of the separated components without the need for additional sample preparation steps. This saves time and resources, making the column an excellent choice for laboratories focused on proteomics research, including peptide mapping, protein identification, and quantification.

The thermostatically controlled column oven ensures consistent temperature conditions during the separation process, contributing to reproducible results. The column is also designed to be easy to use, with a robust and rugged construction that can withstand the demanding conditions of daily use in a laboratory setting.

In summary, the Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC Column is a high-performance liquid chromatography column specifically designed for the reversed-phase separation of peptides and proteins using UHPLC systems. Its compatibility with various MS techniques and NeoCore surface technology make it an excellent choice for proteomics research applications.

Pros and Cons of Buying Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC Column

The Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC column is a popular choice for proteomics and peptide separations. This column comes with several advantages and disadvantages that are essential to consider before making a purchase.

Pros:

1. High Efficiency: This column offers ultra-high performance liquid chromatography (UHPLC) separation, which results in better resolution and increased throughput. The 2.1 mm inner diameter and 100 pore size contribute to efficient and rapid separations.

2. Easy-Spray Technology: This technology enables direct coupling of the UHPLC column to an Orbitrap or Quadrupole mass spectrometer without the need for an additional online pre-column. This simplifies the setup and reduces the risk of contamination, making it a time-saving and cost-effective solution.

3. Compatibility: The Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC column is compatible with various mass spectrometry platforms, including Thermo Scientific's Orbitrap and Quadrupole systems.

4. Robust: The column has a robust design that can handle challenging sample matrices and is resistant to common contaminants, ensuring consistent and reliable separation results.

5. Reproducibility: The column provides excellent reproducibility, ensuring that results from different runs are comparable and consistent.

Cons:

1. Cost: The Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC column is a premium product, and its cost may be a significant investment for some laboratories.

2. Limited Sample Load: Due to its smaller particle size and high efficiency, the column has a lower sample loading capacity compared to standard size particles. This may require users to prepare smaller sample quantities or perform multiple injections for larger samples.

3. Requires Proper Handling: This column requires proper handling, such as avoiding contact with strong acids or bases, and maintaining the recommended temperature and flow rates to ensure optimal performance.

Conclusion:

The Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC column is an excellent choice for laboratories conducting proteomics and peptide separations. Its high efficiency, easy-spray technology, compatibility, robustness, and reproducibility make it a valuable investment for researchers seeking reliable and consistent separation results. However, its cost and limited sample loading capacity may be a concern for some laboratories. Ultimately, the decision to purchase this column depends on the specific needs and resources of the laboratory.

Recommendation:

If your laboratory requires high-performance liquid chromatography for proteomics and peptide separations, the Thermo Scientific Easy-Spray PepMap RSLC C18 2.1 um 100 Neo UHPLC column is a recommended option due to its advantages in separation efficiency, ease of use, and compatibility with various mass spectrometry platforms. However, carefully consider the cost and potential limitations before making a purchase. If budget is a concern, alternative columns with larger particle sizes and lower cost may be a suitable alternative.

Details:

Thermo Scientific ES75750PN EASY-Spray PepMap RSLC C18 2um 100A Neo UHPLC Column.

part #: es75750pn price

  • $1200.00

specifications labequipi:

  • brand: Thermo Scientific
  • mpn: Es75750pn
  • part type: Spray
  • intended use/discipline: Easy-spray Column

offer labequipi:

  • sold: 0
  • started: May 29, 2025
  • availability: In Stock
  • quantity: 2
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  • Healthcare, Lab Dental > Medical Lab Equipment, Devices > Other Medical Lab Equipment
  • condition: Used

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  • time: 30 Days

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part type: spray, intended use/discipline: easy-spray column,
category: business & industrial > healthcare, lab dental > medical lab equipment, devices > medical lab equipment, sku: 17805170310140751,
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